Application of a Two-Dimensional Hadamard Encoding Mask for the Imaging of Thin-Layer Chromatography Plates by Laser-Induced Fluorescence or Surface-Enhanced Raman Scattering and for Use with a Photoacoustic Detector to Generate Three-Dimensional Photoacoustic Images

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Abstract
Spatial imaging of thin-layer chromatography plates using laser-induced fluorescence and surface-enhanced Raman scattering has been accomplished with a stationary two-dimensional Hadamard encoding mask. Detection of three nanograms of pararosaniline hydrochloride on a thin-layer chromatography plate was made possible by means of the surface-enhanced Raman phenomenon. The imaging capabilities of the stationary two-dimensional Hadamard encoding mask coupled with the depth profiling ability of photoacoustic detection allows for the three-dimensional photoacoustic imaging of a multiple analyte sample.

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