Middle repetitive DNA: a fluid component of the Drosophila genome.

Publisher Website
Google Scholar
Abstract
Most of the middle repetitive DNA of D. melanogaster appears to be organized into families of 10-100 repeated elements that are found at scattered locations in the chromosome arms and occupy new chromosomal positions as populations of D. melanogaster diverge. These nomadic DNA segments can be identified by an analysis of cDm plasmids, hybrids of the colicinogenic plasmid ColE1 [from Escherichia coli] and segments of randomly sheared D. melanogaster DNA. cDm plasmids (80) were withdrawn, at random, from a library of approximately 17,000 cDm clones. Of these, 57 seem to contain either DNA that is not repeated in the D. melanogaster genome or DNA that has a low repetition frequency. The remaining 23 cDm plasmids contain repetitive sequences. Of these 23 plasmids, 17 contain repetitive sequences that are demonstrably scattered to many chromosomal sites that can be mapped in 2 D. melanogaster strains, g-1 and g-X11. The repeated elements hybridizing with each of the different Dm segments are at quite different chromosomal locations in these 2 strains. The size of each family of repeated sequences remains fairly constant in both strains. The number of elements in each family may have been fixed by selection.