Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation
- 2 August 2009
- journal article
- research article
- Published by Springer Nature in Nature Chemical Biology
- Vol. 5 (9) , 628-630
- https://doi.org/10.1038/nchembio.196
Abstract
MMP12 is a metalloproteinase implicated in inflammation. To monitor its activity, we synthesized a membrane-targeted reporter (LaRee1) based on Foerster resonance energy transfer (FRET). Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease.Keywords
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