Fundamental Studies on the Evaluation of the Crude Drugs. V : Quantitative Analysis of Constituents in Crude Drugs by Rod-Thin-Layer Chromatography with FID. (2). Determination of Paeoniflorin and Albiflorin in Paeony Roots
Open Access
- 1 January 1977
- journal article
- research article
- Published by Pharmaceutical Society of Japan in YAKUGAKU ZASSHI
- Vol. 97 (8) , 916-921
- https://doi.org/10.1248/yakushi1947.97.8_916
Abstract
A new method for the determination of paeoniflorin and albiflorin in the crude drug Shaoyao (peony [Paeonia] root) was established by the use of a rod-thin-layer chromatograph equipped with a flame ionization detector (rod-TLC-FID). The test solution was submitted to rod-TLC-FID method using a mixture of chloroform-methanol (5:1) as a developing solvent. The content of paeoniflorin and albiflorin was calculated from their calibration curves previously prepared using guaiacol glyceryl ether as an internal standard. The recovery rate of paeoniflorin and albiflorin was more than 95%. This method is useful for the chemical evaluation of Shaoyao.Keywords
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