CHROMOSOME DAMAGE INDUCED BY HYDROXYLAMINE IN MAMMALIAN CELLS

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Abstract
The Chinese hamster cell line 11dFAF28 was used to test the in vitro effects of Hydroxylamine (HA), 5-bromode-oxyuridine (BUDR), and X-rays on mammalian chromosomes. Frequency of breaks occurring in specific regions of chromosomes 1, 2, and X were compared following the three treatments. It was found that chromosomes differ in their total susceptibility to each agent. X-ray damage was found to be random for chromosome 1 and 2 and no regions appeared to be especially sensitive. HA produced breakage at the centromere region of all chromosomes tested. HA has a preferential action on cytosine hence it was suggested that centromeric regions have a high cytosine-guanine content. BUDR treatment produced breakage in the telomeric region of all chromosomes tested. Since regions containing high adenine-thymine ratios are extremely vulnerable to the action of BUDR, it is suggested that the telomeric regions contain large quantities of this base pair.

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