COLONY FORMATION INVITRO BY LEUKEMIC-CELLS IN ACUTE LYMPHOBLASTIC LEUKEMIA (ALL)

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 52  (4) , 712-718
Abstract
An assay system in vitro for the growth of malignant lymphoblastic colony-forming cells (CFC) was established. Growth of malignant myeloblastic CFC was previously reported but this is the 1st report of malignant lymphoblastic CFC growth. Established assay systems in vitro were very helpful in elucidating the control of growth and differentiation of normal and malignant bone marrow cells. Lymphoblastic CFC were grown from the bone marrow aspirates of 20 children with acute lymphoblastic leukemia. Growth of these colonies was established on an agar assay system and maintained in relative hypoxia (7% O2) of a Stulberg chamber. The criteria for malignancy of these colonies was based on cellular cytochemical staining characteristics, specific cell surface markers and the ability of these lymphoid cells to grow without adding a lymphoid mitogen. With this technique, specific nutritional requirements and drug sensitivites can be established in vitro, and these data may permit tailoring of individual antileukemic therapy.

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