The branched respiratory chain of heterotrophically dark‐grown Chloroflexus aurantiacus

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Abstract
The respiratory electron‐transport chain of heterotrophically dark‐grown Chloroflexus aurantiacus has been investigated. Membranes isolated from these cells have been shown to contain at least three c‐type cytochromes (E m, 7.0 255,180, and 10 mV), three b‐type cytochromes (E m, 7.0 of 210, 60 and −65 mV) and two cytochromes of the a type with E m, 7.0 of 330 and 190 mV. Spectroscopic evidence from CO‐difference spectra, CN−‐duference spectra and spectra at fixed oxidation‐reduction potentials suggests that the two a‐type components may be analogous to cytochromes a and a 3 of mitochondria. The analyses of the effects induced by CN−, myxothiazol and antimycin A on both steady‐state respiratory activities and semi‐rapid oxidation‐reduction kinetic patterns of c‐ and a‐type cytochromes indicate the presence of a branched respiratory chain. Growth of Chloroflexus in medium lacking added copper diminished the concentration of the a‐type cytochromes but not those of cytochromes of the b and c type.

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