Does 3-Methyleneoxindole Possess Auxin Activity?

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Abstract
Extensive xylem differentiation was induced in explants of lettuce pith parenchyma cultured for 7 d on Murashige and Skoog's medium containing exogenous auxin and cytokinin. Xylo genesis was not observed by employing various concentrations of 3-methyleneoxindole (MOl) as the source of exogenous auxin. Samples of MOI from two research laboratories were tested in these studies. Tracheary elements were formed in profuse numbers in explants transferred to an IAA-kinetin medium after having been cultured for 7 d on an MOI-kinetin medium. Media containing ethylenediaminetetraacetic acid (EDTA) were inhibitory to xylogenesis whether the auxin employed was IAA or NAA; thus the response was considered not to arise from the chelation of manganous ions involved in the enzymatic oxidation of IAA. Xylogenesis was either unaffected or inhibited by the addition of chiorogenic acid to the medium.

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