A temperature-gradient technique for the elimination of antibiotic resistance

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Abstract
A technique was developed which facilitates the application of elevated temperatures to strains of unknown heat sensitivity during attempts to eliminate antibiotic resistance. Cultures were subjected to a temperature gradient and the lowest temperatures which inhibited growth were found to be highly efficient in the elimination of gentamicin resistance in several strains of Staphylococcus aureus and S. epidermidis.

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