In vitro Culture of Cells Isolated from Dimethylnitrosamine-Induced Renal Mesenchymal Tumors of the Rat

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Abstract
Cells isolated from dimethylnitrosamine (DMN)-induced renal mesenchymal tumors and established in long-term culture retained various properties of malignant cells. In contrast to cells cultured from normal rat kidney cortex, tumor cells appeared to have an unlimited growth potential, were able to establish clones at low seeding rates and to produce colonies in carboxymethyl cellulose (CMC) gel and on chick embryo chorioallantoic membrane (CAM), and were agglutinable by concanavalin A. Cells of tumor lines were quantitatively smaller than normal cells, had a higher nucleus: cytoplasm ratio, a broader range of multiple nucleoli and a higher mitotic index.

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