The Evolution of Oxygen From Illuminated Suspensions of Frozen, Dried, and Homogenized Chloroplasts

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Abstract
O2 was evolved from suspensions of chloroplasts in solns. containing ferric oxalate and potassium ferricyanide. The participation of an enzyme system in the reaction was indicated from the thermo-lability of the prepn. and from the inhibition of low concs. of enzyme poisons. There was in the reaction a prodn. of H + which allowed measurement of reaction velocity. A red filter was used to prevent the effects of light on the reagents at high light intensity. The pH optimum was at 7 or 7.5. Higher rates and more evolution of O2 occurred if N2 rather than air was used in the manometer vessels. temps. of 10[degree]C were employed. Suspensions with potassium ferricyanide alone produced O2 at a lesser rate than when ferric oxalate was also present. Prepns. retained considerable activity after freezing, drying, disintegration of the chloroplasts or pptn. by (NH4)2-SO4.

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