FACTORS WHICH INFLUENCE THE ACTIVITY OF PURIFIED THROMBIN

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Abstract
A correlated study was made of a number of factors which affect the action of thrombin on fibrinogen. Under standardized conditions the clotting time is inversely proportional to thrombin conc. though the correspondence to a simple formula is far from exact. Curves are given to show the effects of temp., electrolyte conc. pH, and colloid content of the clotting mixture. Thrombin prepared from bovine plasma did not clot pig plasma as well as it clotted bovine plasma. However, purified fibrinogen of the 2 spp. is clotted with equal ease. The inhibition is thus due to inhibitory reactions, not to fundamental incompatability of thrombin of the one sp. for the fibrinogen of the other. Suggestions are made regarding the principles to be followed in the assay of thrombin.

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