Electroporation-induced transformation of intact cells of Clostridium perfringens
- 1 September 1988
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 54 (9) , 2322-2324
- https://doi.org/10.1128/aem.54.9.2322-2324.1988
Abstract
Electroporation-induced transformation of intact cells of Clostridium perfringens 3624A with plasmids pAMB1 and pHR106 resulted in 3.8 X 10(-5) and 4.2 X 10(-4) transformants per viable cell, respectively. With respect to shuttle plasmid pHR106, these values represent a greater than 100-fold increase in transformation frequency when compared with the values reported with polyethylene glycol-induced L-phase variants.This publication has 19 references indexed in Scilit:
- Development of a cell wash buffer that minimizes nucleic acid loss from Clostridium perfringens 10543 ACanadian Journal of Microbiology, 1985
- Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.Proceedings of the National Academy of Sciences, 1984
- Shuttle plasmids for Escherichia coli and Clostridium perfringensJournal of Bacteriology, 1984
- Role of DNase in recovery of plasmid DNA from Clostridium perfringensApplied and Environmental Microbiology, 1984
- Stochastic model for electric field-induced membrane pores electroporationBiophysical Chemistry, 1984
- Isolation of a plasmid responsible for caseinase activity in Clostridium perfringens ATCC 3626BJournal of Bacteriology, 1981
- Rapid procedure for detection and isolation of large and small plasmidsJournal of Bacteriology, 1981
- Characterization and transferability of Clostridium perfringens plasmidsPlasmid, 1977
- IDENTIFICATION OF 2 PLASMIDS ISOLATED FROM A BACTERIOCINOGENIC STRAIN OF CLOSTRIDIUM-PERFRINGENS1976
- Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.1972