Reverse Phase Liquid Chromatographic Determination of Aspartame in Beverages and Beverage Mixes
- 1 May 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 67 (3) , 510-513
- https://doi.org/10.1093/jaoac/67.3.510
Abstract
A method is described for determining the artificial sweetener aspartame in beverages and beverage mixes by liquid chromatography. Aspartame is separated on a μC18 column, using a mobile phase of acetic acid, water, and isopropyl alcohol at pH 3.0 and UV detection at 254 nm. Beverages are filtered through 0.45 μm filters and injected directly into the chromatograph. Aspartame is eluted in approximately 7 min. Detection of aspartame is confirmed by a UV scan of the trapped peak. Aspartame is quantitated in the presence of other beverage additives such as saccharin, caffeine, sodium benzoate, artificial colors, and artificial flavors. Results are presented for spiked soda beverages, beverages from fruit-flavored mixes, instant tea, reconstituted presweetened drink mixes, and a powdered tabletop sweetener.This publication has 1 reference indexed in Scilit:
- High Pressure Liquid Chromatographic Determination of Sodium Saccharin, Sodium Benzoate, and Caffeine in Soda Beverages: Collaborative StudyJournal of AOAC INTERNATIONAL, 1979