Functional complementation by electroporation of human BACs into mammalian fibroblast cells
- 15 February 1998
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 26 (4) , 1124-1125
- https://doi.org/10.1093/nar/26.4.1124
Abstract
Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.Keywords
This publication has 11 references indexed in Scilit:
- Homologous recombination based modification in Esherichia coli and germline transmission in transgenic mice of a bacterial artificial chromsomeNature Biotechnology, 1997
- Immortalization of four new fanconi anemia fibroblast cell lines by an improved procedureSomatic Cell and Molecular Genetics, 1996
- Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.Proceedings of the National Academy of Sciences, 1992
- Electroporation in ‘intracellular’ buffer increases cell survivalNucleic Acids Research, 1992
- Chapter 37 Fluorescence in Situ Hybridization with DNA ProbesPublished by Elsevier ,1990
- Comparative anatomy of the human APRT gene and enzyme: nucleotide sequence divergence and conservation of a nonrandom CpG dinucleotide arrangement.Proceedings of the National Academy of Sciences, 1987
- Nucleotide sequence of the human APRT geneNucleic Acids Research, 1987
- Expression of human genes for adenine phosphoribosyltransferase and hypoxanthine-guanine phosphoribosyltransferase after genetic transformation of mouse cells with purified human DNASomatic Cell and Molecular Genetics, 1980
- Assignment of the Gene for Adenine Phosphoribosyltransferase to Human Chromosome 16 by Mouse-Human Somatic Cell HybridizationProceedings of the National Academy of Sciences, 1974
- THE INOSINIC ACID PYROPHOSPHORYLASE ACTIVITY OF MOUSE FIBROBLASTS PARTIALLY RESISTANT TO 8-AZAGUANINEProceedings of the National Academy of Sciences, 1963