Proteins and Peroxidase in Callus and Suspension Cultures of Apple

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Abstract
Different methods of the isolation of soluble proteins were applied to cell cultures of 3 apple cultivars, best results being obtained with a rapid technique based on freezing and thawing. Ultrathin-layer isoelectric focusing followed by an improved Ag staining method showed that proteins from apple callus cultures consist of some 60-80 zones, with isoelectric points mainly between pH 4-7. Depending on protein content, adequate Ag staining is achieved with 50-500 cells. Protein patterns of callus cultures allowed clear discrimination of cultivars. Protein and peroxidase isozyme patterns in cell saps of suspension cultures show striking differences during the growth cycle, whereas the protein patterns from the nutrient media were constant over the entire cultivation period and closely resembled the patterns of stationary phase and callus cells.