Observation of the pre‐steady state process in thermolysin catalysis with a fluorescent displacement probe at low pH
- 3 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 362 (2) , 189-191
- https://doi.org/10.1016/0014-5793(95)00242-2
Abstract
The pre-steady state process in the thermolysin-catalyzed hydrolysis of Cbz-Gly-Phe-Ala was observed at pH 4.5 by fluorescence stopped-flow method using Dns-Phe as a displacement probe. After the confirmation of the pre-equilibrium hypothesis for the binary interaction, the nonlinear substrate concentration dependence of the apparent kinetic constant for the pre-steady state process was analyzed and an existence of multi-intermediates was proposedKeywords
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