A Direct Preparative High Performance Liquid Chromatography Procedure for the Isolation of Major Triterpenoids and Their Quantitative Determination in Neem Oil

Abstract
Isolation of the major triterpenoids from neem oil in pure form by a direct preparative High performance liquid chromatography procedure is presented. As neem oil is an important component of Neem based insecticidal formulations, a simple, isocratic analytical HPLC procedure for quantitation (relative abundance) of these major bioactive triterpenoids has been developed.