P2Y isoforms operative in mouse taste cells

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Abstract
Recent functional evidence indicates that mouse taste cells express P2Y receptors coupled to IP3 production and Ca2+ mobilization. Our studies of the expression profile of particular P2Y isoforms in the taste tissue of the mouse have revealed that ATP and UTP equipotently mobilize intracellular Ca2+ at saturating concentrations, suggesting that common receptors for both nucleotides, i.e., P2Y2 and P2Y4 subtypes, might be involved. Reverse transcription/polymerase chain reaction and immunohistochemistry have confirmed the presence of P2Y2 and P2Y4 receptors in a population of taste bud cells from the circumvallate and foliate papillae. Transcripts for the P2Y1 and P2Y6 isoforms have also been detected in taste tissue preparations, this observation being consistent with the ADP and UDP responsiveness of taste cells. Together, our data suggest that P2Y2 and P2Y4 receptors play a predominant role in mediating taste cell responses to ATP and UTP.