FACTORS THAT INFLUENCE INITIATION AND GROWTH OF 9L RAT-BRAIN GLIOSARCOMA MULTICELLULAR SPHEROIDS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (4) , 1223-1226
Abstract
Optimum conditions for initiating and growing 9L rat brain gliosarcoma multicellular spheroids are detailed. Best results were achieved when 0.5-2.0 .times. 106 cells were seeded initially into agarose (0.5-1.0%)-coated Petri dishes. Spheroids initiated in this manner appeared to grow in spinner culture at a rate slightly faster than spheroids initiated and grown in spinner culture. An optimum growth rate was obtained for individual spheroids grown in the wells of cluster dishes coated with 0.5-0.75% agarose in growth medium containing 10% newborn calf serum and 0.9 g glucose/l. Three methods for dissociation of spheroids at both room temperature and 37.degree. C were developed. The race track method of fractional dissociation produced a constant and similar number of cells for each disaggregation interval, and the plating efficiency of cells in each fraction was similar.

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