High Resolution Two-dimensional Polyacrylamide Electrophoresis of Human Serum Proteins
Open Access
- 1 February 1972
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 57 (2) , 173-185
- https://doi.org/10.1093/ajcp/57.2.173
Abstract
A two-dimensional polyacrylamide gel electrophoretic technic in which serum is first separated in a soft gel column followed by electrophoresis in a 2 to 30% linear gradient gel slab is described. An apparatus which is simple in design and offers ease of operation to assure reproducible protein patterns is described. More than 100 normal serum protein fractions could be demonstrated in normal as well as pathologic serum specimens, including several small molecules presumed to be serum peptides. The serum components identified to date include: immunoglobulins G, A, and M; β2-lipoprotein; α2-macroglobulin; haptoglobin; ceruloplasmin; transferrin; α1-acid glycoprotein; albumin; and pre-albumin. Several post- and pre-albumin components were detected in sera from patients with myeloma, leukemia, and Hodgkin’s disease but were not detected in sera of normal subjects.Keywords
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