Involvement of phosphatidylinositol 3‐kinase in nuclear translocation of protein kinase C ζ induced by C2‐ceramide in rat hepatocytes
- 16 February 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 514 (2-3) , 361-365
- https://doi.org/10.1016/s0014-5793(02)02401-8
Abstract
In this study we report that protein kinase C ζ (PKC ζ), one of the atypical isoforms of the PKC family located predominantly in cytosol, is redistributed by C2-ceramide treatment in isolated hepatocytes. PKC ζ increased in membrane and nuclear fractions after 30 min of treatment with C2-ceramide in a dose- and time-dependent manner. The action of C2-ceramide was inhibited by wortmannin and LY 294002, indicating that C2-ceramide-induced PKC ζ increase in both nucleus and membrane fractions is mediated by phosphatidylinositol 3-kinase (PI3-kinase) activation. In addition, a significant translocation of PI3-kinase to the nucleus was observed after C2-ceramide treatment.Keywords
This publication has 35 references indexed in Scilit:
- Phosphoinositide 3-Kinase Regulates Phospholipase Cγ-mediated Calcium SignalingJournal of Biological Chemistry, 1998
- Phosphoinositide 3-Kinase in Rat Liver NucleiBiochemistry, 1998
- Activation of Phospholipase C-γ by Phosphatidylinositol 3,4,5-TrisphosphateJournal of Biological Chemistry, 1998
- Sphingolipids Increase Calcium Concentration in Isolated Rat Liver NucleiBiochemical and Biophysical Research Communications, 1997
- Chromatin Neutral Sphingomyelinase and Its Role in Hepatic RegenerationBiochemical and Biophysical Research Communications, 1997
- Ceramide Inactivates Cellular Protein Kinase CαJournal of Biological Chemistry, 1996
- Signal transduction through lipid second messengersCurrent Opinion in Cell Biology, 1996
- PCA-4248, a PAF receptor antagonist, inhibits PAF-induced phosphoinositide turnoverEuropean Journal of Pharmacology: Molecular Pharmacology, 1995
- Redistribution of Protein Kinase C Isoforms in Human Neutrophils Stimulated by Formyl Peptides and Phorbol-Myristate AcetateBiochemical and Biophysical Research Communications, 1995
- Regulation of Atypical ζ-Protein Kinase C in Cellular SignalingExperimental Cell Research, 1994