Time-resolved fluorometry in nucleic acid hybridization and Western blotting techniques
- 1 January 1993
- journal article
- review article
- Published by Wiley in Electrophoresis
- Vol. 14 (1) , 866-875
- https://doi.org/10.1002/elps.11501401138
Abstract
Time‐resolved fluorometry is now used extensively for immunological assays and to a lesser extent in other research areas. In this review I describe applications of time‐resolved fluorometry in nucleic acid hybridization and in blotting techniques, including Southern and Western blotting. Clearly, timeresolved fluorometry has potential for playing a major role in techniques other than immunological assays.Keywords
This publication has 24 references indexed in Scilit:
- Quantitative Western blot analysis and spot immunodetection using time-resolved fluorometryJournal of Immunological Methods, 1992
- Detection of Lanthanide Chelates by Time-Resolved FluorescencePublished by Elsevier ,1992
- Analytical methodology for immunoassays and DNA hybridization assays — current status and selected systems — critical reviewClinica Chimica Acta; International Journal of Clinical Chemistry, 1990
- Europium Chelate Labels in Time-Resolved Fluorescence Immunoassays and DNA Hybridization AssaysAnalytical Chemistry, 1990
- Detection techniques for immunoassay and DNA probing applicationsClinical Biochemistry, 1990
- Multiple fluorescence labeling with europium chelators. Application to time-resolved fluoroimmunoassaysAnalytical Chemistry, 1989
- DNA Diagnostics—Molecular Techniques and AutomationScience, 1988
- Time-resolved fluorescence using a europium chelate of 4,7-bis-(chlorosulfopheny1)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA)Journal of Immunological Methods, 1988
- A new europium chelate for protein labelling and time-resolved fluorometric applicationsClinical Biochemistry, 1988
- Immunoassays with time-resolved fluorescence spectroscopy: Principles and applicationsClinical Biochemistry, 1988