Thrombin stimulates Na+‐H+ exchange across the human platelet plasma membrane
- 9 July 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 172 (2) , 272-274
- https://doi.org/10.1016/0014-5793(84)81139-4
Abstract
We have investigated the release of protons from thrombin‐stimulated platelets. Addition of thrombin to suspensions of washed platelets resulted in fast liberation of H+. In the presence of 0.1 mM amiloride, a potent inhibitor of the Na+/H+ transport system, the amount of protons liberated was decreased by about 50%, and was further reduced to about 15% by 1 mM amiloride. Similar inhibition of H+ release was observed after Na+ in the incubating medium had been replaced by choline. We conclude that one of the earliest events in thrombin‐stimulated platelets consists of the activation of an Na+/H+ countertransport, which leads to an increase in intracellular pH.Keywords
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