HEMATOPOIETIC STEM-CELL DYNAMICS IN SR-89 MARROW-ABLATED MICE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 89  (3) , 592-602
Abstract
89Sr was used to ablate the marrows of 12-16 wk old CAF1 mice. The CFU-S [spleen colony forming unit] of their blood, marrows, and sleens were assayed at intervals from days 10-56. The effects of splenectomy performed on day 14 or 42 on the numbers of CFU-S in the blood and marrow were also studied. On day 10 after treatment with 89Sr, both the cellularity and CFU-S of the marrow were markedly decreased. Later, especially during the 3rd wk after treatment, marrow cellularity increased and by day 56 had returned to 74% of normal; the concentration of marrow CFU-S also increased but by day 56 had attained a level of only 1/3 normal. Replenishment of the marrow''s CFU-S lagged behind the repletion of its cellularity. Spleen and blood CFU-S were elevated throughout the 56 days. The number of splenic CFU-S was highest at day 10, decreased somewhat by day 21 and remained remarkably stable thereafter. After splenectomy there was a significant decline in the content of both blood and marrow CFU-S, but the reverse occurred in the cold 89Sr-treated control group. In the 89Sr-irradiated animal, the spleen is apparently transformed from a trapper to the prime supplier of CFU-S and in normal mice, the spleen may suppress marrow CFU-S proliferation. An inverse relationship between the size of the pool of mature granulocytes and the number of CFU-S was found, suggesting that the granulocyte compartment may, at least in part, play a role in the regulation of CFU-S proliferation.

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