Reactivation of folate-binding protein from cow's milk, purified by affinity chromatography in the presence of lecithin and other surfactants
- 1 September 1981
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 1 (9) , 721-726
- https://doi.org/10.1007/bf01116470
Abstract
Purification by affinity chromatography of the folate-binding protein from cow''s milk leads to a drastic lowering of the affinity of the protein for folate. The purified protein was completely reactivated in the presence of a number of surfactants (phospholipids and synthetic detergents) particularly of cationic or non-ionic type. There is a striking analogy between the present results and the well-known lipid/detergent reactivation of certain purified membrane-derived enzymes.This publication has 15 references indexed in Scilit:
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