Influence of Amount of Test Virus on Quantitative in ovo Canine Distemper Virus Neutralization Test

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Abstract
Under precisely controlled conditions, serum canine distemper (CD) neutralizing antibody titers were determined against 10-fold increments of CD virus after both 2 hours and 18 hours of incubation of serum-virus mixtures. The resulting data do not support the existence of the reported inverse linear slope relationship between log CD virus concentration and log CD neutralizing antibody titer. Whereas observed titers were not materially affected within a range of 100 to 1, 000 virus EID50 for the 18-hour determinations, and 100 to 10,000 EID50 for the 2-hour determinations, "antibody units" calculated by a proposed method of mathematical interpolation were not in accord with these observed titers. The data have a practical bearing on standardization of the test, as well as attempts to make test results of different laboratories more directly comparable.

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