L-Serine Production by Methanol-utilizing BacteriumPseudomonasMS 31

Abstract
A methanol-utilizing bacterium, which produced red to pink pigments and assimilated methanol via icl- serine pathway, was isolated from soil and tentatively designated as Pseudomonas MS 31. This bacterium produced L-serine when glycine was added to the growth medium at the late exponential phase of growth. The cells showed high L-serine degradation activity. Chelating agents and some metal ions, which inhibited L-serine degradation, stimulated the L-serine accumulation. In the presence of 0.1 ˜ 1 mM EDTA, o-phenanthroline, 8-hydroxyquinoline, α,α'-dipyridyl, cobalt sulfate or nickel sulfate, this bacterium produced 0.7˜2.1mg L-serine from 4mg glycine per ml culture.

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