Characterization and Translation Studies of Potato Virus S RNA
- 1 January 1990
- journal article
- research article
- Published by Scientific Societies in Phytopathology®
- Vol. 80 (5) , 441-445
- https://doi.org/10.1094/phyto-80-441
Abstract
The Mr of the Andean (An) strain of potato virus S (PVS) RNA was determined to be 2.4 .times. 106 (7.5 kilobases [kb]) when assayed by electrophoresis under denaturing conditions. Oligo d(T) cellulose chromatography and direct RNA sequencing demonstrated that viral RNA is polyadenylated. In vitro translation in rabbit reticulocyte lysates indicates that four major protein products of 140K, 123K, 104K, and 34K are produced by PVS-An RNA. Antisera specific to the Andean and type strains of PVS immunoprecipitated a major protein product of 34K. Infection of potato protoplasts indicated that production of polypeptide of 34K, which was not detected in mock inoculated protoplasts and reacted specifically with PVS-An antisrea in immunoprecipitation experiments.This publication has 2 references indexed in Scilit:
- Complete nucleotide sequence of brome mosaic virus RNA3Journal of Molecular Biology, 1981
- Cell Membrane Antigen Isolation with the Staphylococcal Protein A-Antibody AdsorbentThe Journal of Immunology, 1976