High Performance Liquid Chromatographic Analysis of Drugs. I. Simultaneous Determination of Dexamethasone and Chlorpheniramine Maleate in Ointment

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Abstract
Chlorpheniromine moleate (MCP) and dexamethasone (DM) are used in a variety of dermatological disorders. Simultaneous determination of MCP and DM in ointment was established by using high performance liquid chromatography. On a .mu.-Bondapak C18 column (4 mm i.d. internal diameter .times. 300 mm) with an eluent of CH3OH: H2O = 80 : 20, sufficient separation of MCP, DM and other components in ointment was obtained. MCP and DM in ointment were extracted with CH3OH. A 2 .mu.l portion of the extracted solution with naphthalene as internal standard was injected into the column, and the effluent was monitored by UV detector (.lambda. = 254 nm). Coefficients of variation for the determination of MCP and DM in ointment were found to be less than 0.35%. Compared to other methods (determination of DM with isoniazid dihydrochloride spectrophotometric determination of MCP by solvent extraction with zincon), the present method was superior in simplicity and accuracy.

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