Electron microscopic study on vaccinia virus release

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Abstract
Summary FL cells infected with the IHD-W strain of vaccinia virus were studied by scanning and transmission electron microscopy. A large number of naked virus particles were found to accumulate beneath the host cell plasma membrane and to protrude from the cell surface. It was seen in some cases that naked viral particles were released by budding not only from the cell surface but also from the surface of cytoplasmic packets which were seen along the cell periphery.