Comparison of high performance liquid cliromatographic and microbiological methods for determination of itraconazole
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 21 (1) , 93-100
- https://doi.org/10.1093/jac/21.1.93
Abstract
A reversed-phase high-performance liquid chromatographic (HPLC) method, with internal standard quantification, is described for the analysis of itraconazole in human serum. No interference was encountered from over 60 drugs tested. The standard curve was linear from 10 to 10000 μg/l. The detection limit of the method was 10 μg/l, with coefficients of variation from 2·2 to 7·8% over a range of itraconazole concentrations from 20 to 1600 μg/l. An agar diffusion method is also described with a lowest reproducible limit of 100 μg/l. This method had coefficients of variation from 11·0 to 17·1% over a range of itraconazole concentrations from 100 to 1600 μg/l. Comparison of the methods showed that HPLC gave much lower values of itraconazole concentrations in patient serum samples than did the microbiological method.Keywords
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