Utilisation d'inogula preculttves dans les essais de biodegradabilite the use of precultured inocula for biodegradability tests

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Abstract
The biodegradation of benzolc acid, pentaerythritol and para‐nitrophenol was investigated in a die‐away closed bottle test with sixteen different inocula (8 activated sludges and 8 river waters). Each of them was used just after collection or after continuous culture with acetate (acclimatation) and pentaerythritol or para‐nitrophenol as the only source of carbon and energy (adaptation). Acclimation or adaptation of the inocula reduces the initial variability between them in term of biomass (coefficient of variation between 101 and 257% before preculture and between 27 and 74% after preculture). However, only adapted inocula allowed biodegradation assays to be homogeneous in their results for pentaerythritol and para‐nitrophenol while preculture of the inoculum is not necessary for an easily biodegradable substance like benzolc acid. Our results show that preculture of the inocula with the xenobiotic (adaptation) within defined conditions (continuous culture) resolves the problem of the lack of reproducibility between biodegradability tests, decreases the lag time and increases the rate of biodegradation.