Chloramphenicol Acetylation in Whole Cells of Escherichia coli Carrying R-Factors Characterization and Kinetic Studies

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Abstract
Chloramphenicol resistance has been shown to be transferable in 80 out of 95 wild type strains of E. coli. A new sensitive test (by thin layer chromatography) demonstrated that all these resistant strains acetylate the drug. No acetylation of chloramphenicol was detected in sensitive wild type strains of E. coli or in sensitive and resistant strains of E. coli K12. Kinetic studies with whole cells showed that acetylation requires an exogenous source of energy like glucose rather than acetylcoenzyme A; the cellular reserve of the latter seems to be sufficient for the reaction.

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