ANALYSIS OF PLANT EXTRACTS FOR CHLOROPHYLLS a AND b BY A PHOTOELECTRIC SPECTROPHOTOMETRIC METHOD
Open Access
- 1 April 1942
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 17 (2) , 198-209
- https://doi.org/10.1104/pp.17.2.198
Abstract
A simple, rapid, and accurate spectroscopic method for the deter-mination of chlorophylls a and b in plant materials is described. An acetone extract is made from fresh leaf material. The fat-soluble pigments are transferred to ether, the acetone removed, and the solution dried with Na2SO4. Spectroscopic analysis for chlorophyll a and chlorophyll b is made at wavelengths 6600 and 6425 A. The reliability of values obtained from other wavelengths depends considerably upon the carotenoid content. The chemical precautions and instrumental conditions essential for quantitative results have been discussed. The method has been applied to 12 plant sources.This publication has 1 reference indexed in Scilit:
- SPECTROSCOPIC ANALYSIS OF PLANT EXTRACTS FOR CHLOROPHYLLS a AND bPlant Physiology, 1941