Stereochemistry of Peroxisomal and Mitochondrial β‐oxidation of α‐methylacyl‐CoAs
Open Access
- 1 March 1997
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 244 (2) , 434-440
- https://doi.org/10.1111/j.1432-1033.1997.00434.x
Abstract
The stereochemistry of β‐oxidation of α‐methyl‐branched fatty acids was analyzed, in rat liver and in human cells, with (2R)‐ and (2S)‐2‐methyltetradecanoic acid as model substrates. In rat liver, formation of the α,β‐unsaturated compound was found to be concentrated in mitochondria while in human cells, this activity co‐distributed mainly with peroxisomal marker enzymes. In both cases, the dehydrogenating enzymes were absolutely specific for the (2S)‐enantiomer. In human liver, activation was some three times faster with the (2R)‐ than with the (25)‐isomer while in rat liver both were activated at about the same rate.Keywords
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