Lactic acid bacteria secrete metabolites retaining anti-inflammatory properties after intestinal transport
Top Cited Papers
Open Access
- 1 June 2004
- Vol. 53 (6) , 821-828
- https://doi.org/10.1136/gut.2003.026252
Abstract
Background: Probiotic bacteria have a beneficial effect on intestinal inflammation. In this study, we have examined the effect of lactic acid and commensal Gram positive (+) bacteria conditioned media (CM) on tumour necrosis factor α (TNF-α) release and the mechanisms involved. Methods: Lipopolysaccharide (LPS) induced TNF-α secretion by peripheral blood mononuclear cells or the THP-1 cell line was monitored in the presence or absence of bacteria CM obtained from two probiotic strains, Bifidobacterium breve (Bb) and Streptococcus thermophilus (St), and three commensal bacterial strains (Bifidobacterium bifidum, Ruminococcus gnavus, and unidentified Streptococcus). Bb and St bacteria CM were allowed to cross filter grown intestinal epithelial cell monolayers (HT29-19A) to assess intestinal transport of active bacterial products. These products were characterised and their effect on LPS binding to THP-1 cells and nuclear factor κB (NFκB) activation assessed. Results: Dose dependent inhibition of LPS induced TNF-α secretion was noted for both probiotic bacteria CM (64% and 71% inhibition for Bb and St, respectively) and to a lesser extent commensal bacteria CM (21–32% inhibition). Active products from Bb and St were resistant to digestive enzymes and had a molecular mass Bb and St CM. Conclusion:B breve and S thermophilus release metabolites exerting an anti-TNF-α effect capable of crossing the intestinal barrier. Commensal bacteria also display a TNF-α inhibitory capacity but to a lesser extent. These results underline the beneficial effect of commensal bacteria in intestinal homeostasis and may explain the role of some probiotic bacteria in alleviating digestive inflammation.Keywords
This publication has 30 references indexed in Scilit:
- Ruminococcin A, a New Lantibiotic Produced by a Ruminococcus gnavu s Strain Isolated from Human FecesApplied and Environmental Microbiology, 2001
- Prokaryotic Regulation of Epithelial Responses by Inhibition of IκB-α UbiquitinationScience, 2000
- Butyrate inhibits inflammatory responses through NFkappa B inhibition: implications for Crohn's diseaseGut, 2000
- Transforming Growth Factor-β Inhibits Lipopolysaccharide-Stimulated Expression of Inflammatory Cytokines in Mouse Macrophages through Downregulation of Activation Protein 1 and CD14 Receptor ExpressionInfection and Immunity, 2000
- The luminal short-chain fatty acid butyrate modulates NF-κB activity in a human colonic epithelial cell linePublished by Elsevier ,2000
- Lactobacillus species prevents colitis in interleukin 10 gene–deficient miceGastroenterology, 1999
- LPS-binding protein protects mice from septic shock caused by LPS or gram-negative bacteria.Journal of Clinical Investigation, 1998
- Intestinal epithelial cells down-regulate macrophage tumor necrosis factor-alpha secretion: A mechanism for immune homeostasis in the gut-associated lymphoid tissueSurgery, 1995
- Evidence for a CD14+ population of monocytes in inflammatory bowel disease mucosa—implications for pathogenesisClinical and Experimental Immunology, 1995
- Differential expression of transforming growth factors alpha and beta in rat intestinal epithelial cells.Journal of Clinical Investigation, 1989