Studies on LM protein appearing in submandibular glands of isoproterenol-treated rats. I. Purification and characteristics of its appearance.

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Abstract
A large mobile (LM) protein that accumulates in rat saliva upon chronic administration of isoproterenol (IPR), a .beta.-adrenergic drug was purified to electrophoretic homogeneity. Rabbit anti-LM protein antibody was prepared and used for determination of the LM protein by a single radial immunodiffusion method. The increase in the amount of this protein in the soluble fraction of submandibular glands was roughly proportional to the increase in the wet weight of the glands and the most effective dose of IPR was 40 mg/kg. The LM protein which accumulated in the submandibular glands upon chronic administration if IPR was secreted as a result of IPR stimulation, whereas methoxamine, an .alpha.-adrenergic drug, did not significantly induce secretion of the accumulated LM protein. The newly synthesized protein in submandibular glands upon chronic administration of IPR is secreted through the action of .beta.-adrenergic receptors.