Passive Administration of Antiserum and Complement in Producing Anti-EL4 Cytotoxic Activity in the Serum of C57BL/6 Mice

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Abstract
Experiments were performed to determine what parameters were essential to develop circulating cytotoxic anti-EL4 activity in normal C57BL/6 mice after transfer of heterologous anti-EL4 serum. When heterologous anti-EL4 serum was passively administered to normal C57BL/6 mice, circulating activity sufficient to lyse EL4 lymphoma cells in vitro was acquired only with the concurrent passive administration of heterologous complement. Evidence for the presence of such circulating in vivo activity was the demonstration of cytotoxic activity in the sera of such mice when assayed directly in vitro against EL4 cells without the in vitro addition of autologous or heterologous complement. Endogenous host complement was unable to mediate tumor cell lysis in vitro, and serum harvested from mice receiving antibody alone lacked the ability to lyse EL4 cells acquired by other mice receiving both heterologous antibody and complement. During examination of the homology and cell-specificity requirements of both autologous complement and other complement sources in reacting optimally with heterologous antiserum, an in vitro tumor cytotoxicity assay appeared better able to predict the ability of passively transferred complement in mediating circulating cytotoxicity for EL4 cells than the standard hemolytic assay.

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