Isolation of Human T Lymphocytes by a Negative Selection Procedure
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Immunological Communications
- Vol. 8 (1) , 49-54
- https://doi.org/10.3109/08820137909044705
Abstract
A single step method to isolate human T [thymus-derived] lymphocytes was achieved using a rosette technique to deplete non-T lymphocytes. Erythrocytes coated simultaneously with Ig[immunoglobulin]G and complement were used to form rosettes with cells bearing such receptors during a short incubation period and the rosetting cells removed by gradient centrifugation. T lymphocytes were isolated (> 96% T cells) without much experimental manipulation and in good recovery (mean 60%, range 40-75%).This publication has 9 references indexed in Scilit:
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