A Chemically Defined Medium for the Growth and Synthesis of Ergot Alkaloids by Species of Balansia

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Abstract
A chemically defined medium was developed for the growth of an ergot alkaloid synthesis by 5 spp. of Balansia (Clavicipitaceae) [B. epichloe, B. henningsiana, B. claviceps, B. strangulans, B. obtecta]. It was determined that the N required for growth was not strict, but NH4Cl (2.4 g/l) produced good growth and favored total ergot alkaloid synthesis. The fungi required thiamine, nicotinic acid, and pyridoxine for growth. Incorporating DL-tryptophan into the medium did not increase growth, but did cause a 2-4 fold increase in the yield of total alkaloid produced by the species tested.

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