The Effect of Human Serum on 3 H-Thymidine Incorporation in Human Prostate Tumors in Tissue Culture

Abstract
Human prostatic tumors, 4 adenomas and 4 adenocarcinomas, were established in tissue culture. Incorporation of 3H-thymidine into DNA was used as a measure of cell activity to compare the effects of supplementing the culture media with human or fetal calf serum. 3H-thymidine incorporation was greater with human serum, with up to 120% increase observed. The addition of dihydrotestosterone (10-8M) did not enhance or inhibit 3H-thymidine incorporation.