Cytotoxic T cell response against lymphoblasts infected with Moloney (Abelson) murine leukemia virus. Methodological aspects and H‐2 requirements

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Abstract
A method for infection of lymphocytes with Moloney(Abelson) murine leukemia virus [M(A)‐MuLV] is described. Only lymphoblasts obtained after stimulation of normal spleen cells by the B cell mitogen lipopolysaccharide (LPS) were satisfactory targets for virus‐specific, secondary cytotoxic T lymphocytes (CTL), whereas spleen cells stimulated by the T cell mitogen concanavalin A were not. The secondary CTL response against M(A)‐MuLV could be efficiently measured using M(A)‐MulV‐infected LPS blasts as stimulating cells for secondary in vitro restimulation and as target cells for virus‐specific destruction. Cold target inhibition demonstrated virus specificity of CTL. The T cell character of the cytotoxic cells was demonstrated by their sensitivity to anti‐Thy‐1.2 treatment. Using syngeneic virus‐infected LPS blasts as target and stimulator, CTL responses were measured with effector cells from C57BL mice of the H‐2b haplotype and of recombinant haplotypes sharing either K or D alleles with H‐2b. In analogy with previous studies on Moloney virus‐specific CTL. it was observed that C57BL/6 (H‐2b) effector cells predominantly lysed Db‐compatible, virus‐infected target cells; B10.A(5R), (KbDd) effector cells showed a poor CTL response against syngeneic, virus‐infected target cells. The combined findings indicate the existence of an Ir gene in the H‐2D region regulating the CTL response against Moloney leukemia virus.

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