Cell surface properties of HLA antigens on Epstein-Barr virus-transformed cell lines.

Publisher Website
Google Scholar
Abstract
A number of monoclonal antibodies were used to investigate the distributions and rates of lateral motion of the HLA-A, B and -DR antigens on several Epstein-Barr virus-transformed human B cell lines. The lateral diffusion coefficients (D) of fluorescein conjugates of the monoclonal antibodies bound to the cell surface were determined by fluorescence recovery after pattern photobleaching. D of HLA-A and -B were found to be comparable and of the order of 10-9-10-10 cm2/s for each of the 7 monoclonal antibodies and 4 cell lines examined. The HLA antigens appear to be monomeric on the cell surface based on experiments using mixtures of arsanilic acid-conjugated and fluorescein-conjugated antibodies. Four monoclonal antibodies against DR antigens were examined. Two of these, Genox 3.53 and L243, labeled the cell surface uniformly and gave D comparable to those obtained for the HLA-A and -B antigens. The other 2, DA2 and 2.06, rapidly patched on the cell surface and were immobile. The DA2, L243 and Genox 3.53 antibodies bound outside of the caps formed with the arsanilic acid-conjugated 2.06 antibody and a 2nd-step rhodamine-conjugated rabbit anti-arsanilate antibody. There appear to be multiple distinct antigens coded for by the HLA-DR region.