Construction of recombinant plasmids containing rat muscle actin and myosin light chain DNA sequences.
- 1 February 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (2) , 960-964
- https://doi.org/10.1073/pnas.77.2.960
Abstract
The construction and partial characterization of recombinant bacterial [Escherichia coli] plasmids carrying DNA sequences that hybridize with rat skeletal muscle actin and a myosin L chain mRNA is described. DNA of 1 clone hybridizes specifically with the muscle-specific .alpha.-actin mRNA. Three plasmid clones contain DNA inserts that hybridize with muscle and with nonmuscle actin mRNA. A 5th plasmid contains sequences complementary to mRNA coding for myosin L chain 2. DNA of this plasmid hybridizes specifically with RNA extracted from muscle and differentiated muscle cultures but not with RNA extracted from proliferating mononucleated myogenic cells.Keywords
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