Protection of Actin from Heat Denaturation by Various Phallotoxins
- 1 January 1976
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 357 (2) , 1139-1144
- https://doi.org/10.1515/bchm2.1976.357.2.1139
Abstract
Phallotoxins protect F-actin [of rabbit skeletal muscle] from heat denaturation at a temperature of 70.degree. C for 3 min. This was shown by difference spectroscopy. G-actin was not protected from heat denaturation by phalloidin. Four toxic phallopeptides showed the same protecting ability; non-toxic seco-compounds had no effect. The non-toxic (S)-phalloidinsulfoxide. A exhibited only a partial protecting activity, corresponding to an affinity for F-actin of about 0.1 of that of the toxic peptides. Evidence for this was obtained from the easy displacement of the sulfoxide A from F-actin by phalloidin and by a spectroscopic dilution titration.This publication has 2 references indexed in Scilit:
- Poisonous Principles of Mushrooms of the Genus AmanitaScience, 1968
- Enzymic nature of F-actin at high temperatureJournal of Molecular Biology, 1966