Purification and characterization of calmodulin from sea urchin spermatozoa
- 1 July 1980
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 59 (2) , 377-381
- https://doi.org/10.1530/jrf.0.0590377
Abstract
Calmodulin was purified to apparent homogeneity from sea urchin spermatozoa by heat-treatment at 85.degree. C, ammonium sulfate precipitation at pH 4.2, DEAE-Sephacel chromatography and gel filtration on Sephadex G-100. Approximately 8.3 .mu.g calmodulin were recovered per 1010 sperm cells. The sperm calmodulin had an apparent MW of 17,800. The purified calmodulin activated calmodulin-deficient phosphodiesterase from pig coronary arteries, with 1/2 maximal activation occurring at approximately 40 ng calmodulin/ml. Trifluoperazine inhibited the sperm calmodulin activity. Evidently calmodulin is present in high amounts in sea urchin spermatozoa, and is essentially the same as the calmodulin isolated from various other tissues.This publication has 8 references indexed in Scilit:
- Calcium-binding modulator protein from the unfertilized egg of the sea urchin Arbacia punctulata.The Journal of cell biology, 1979
- High levels of a calcium-dependent modulator protein in spermatozoa and its similarity to brain modulator proteinBiochemical and Biophysical Research Communications, 1978
- Modulator protein as a component of the myosin light chain kinase from chicken gizzardBiochemistry, 1978
- Partial purification of the Ca2+-Mg2+ ATPase activator from human erythrocytes: Its similarity to the activator of 3′:5′ — cyclic nucleotide phosphodiesteraseBiochemical and Biophysical Research Communications, 1977
- Phosphodiesterase protein activator mimics red blood cell cytoplasmic activator of (Ca2+-Mg2+)ATPaseBiochemical and Biophysical Research Communications, 1977
- Structural similarities between the Ca2+-dependent regulatory proteins of 3':5'-cyclic nucleotide phosphodiesterase and actomyosin ATPase.Journal of Biological Chemistry, 1976
- Nucleoside 3′,5′-Monophosphate Phosphodiesterases in Sea Urchin Sperm1Biology of Reproduction, 1976
- MECHANISM BY WHICH PSYCHOTROPIC-DRUGS INHIBIT ADENOSINE CYCLIC 3',5'-MONOPHOSPHATE PHOSPHODIESTERASE OF BRAIN1976