EFFECT OF ADRIAMYCIN AND RADIATION ON G-2 PROGRESSION

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (9) , 3212-3216
Abstract
The effect of the DNA-intercalating [antitumor] antibiotic adriamycin on the progression of Chinese hamster ovary cells into mitosis, and the delay induced by ionizing radiation, was studied using the mitotic cell selection procedure to monitor the rate of cell division. Following the addition of adriamycin, the mitotic rate remained unaltered for a refractory period and then decreased to zero. This effect was concentration dependent with transition points between the S-G2 boundary for 0.1 .mu.g/ml and late G2 for 250 .mu.g/ml. Cells treated with a 10- or 30 min pulse of 1.0 .mu.g adriamycin per mil exhibited a refractory period identical to that observed for continuous treatment. After a delay of .simeq. 3.5 or .simeq. 5 h, respectively, cell division resumed. The mitotic rate of cells that received 150 rad of X-ray at the onset of an adriamycin pulse declined coincident with that of radiation only, but resumed coincident with those receiving adriamycin only. This implies that radiation induced division delay (.simeq. 3 h) was repaired before cells recovered from adriamycin-induced division delay, and the 2 agents were not additive. This lack of synergism is in contrast to that observed for cell lethality.

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