Expression of the IL-2 receptor γ chain on various populations in human peripheral blood

1 August 1994

journal article
Published by Oxford University Press (OUP) in International Immunology

Vol. 6 (8) , 1273-1277
https://doi.org/10.1093/intimm/6.8.1273

Abstract

We have established two rat mAbs, TUGH4 and TUGh5, specific for the human γ chain of the IL-2 receptor (IL-2R), which is known to be shared among receptors for IL-2, IL-4 and IL-7. The antibodies bound to cell lines transfected with the human γ chain gene but not to their parental cell lines, and precipitated 65–70 and 80–90 kDa cell surface molecules from lysates of human T cells surface-labeled with Na¹²⁵I and chemically cross-linked with ^[125]IL-2 respectively. Flow cytometry with TUGh4 and TUGh5 detected the γ chain in a wide variety of peripheral blood cell populations including CD4⁺ T cells, CD20⁺ T cells, CD20⁺ B cells, CD56⁺ natural killer cells, CD4⁺ monocytes and granulocytes, contrasting with expression of the α and β chains of IL-2R.

Keywords

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