SCREENING OF ANTI-B CELL ANTIBODIES IN SERA OF RENAL-TRANSPLANT RECIPIENTS BY USING DAUDI CELL LINE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 48  (4) , 321-325
Abstract
To examine the presence of antibodies against B [bone marrow derived] cells, 83 sera obtained from 55 renal transplant recipients were screened by the microcytotoxicity test using the Daudi [human Burkitt''s lymphoma lymphoblastoid] cell line. Of 83 samples, 12 were pretransplant sera, 62 were post-transplant sera and 9 were from patients whose grafts were removed due to rejection. To improve the reliability of the tests, 5 different rabbit complements were selected and cytotoxicity tests with them were carried out simultaneously. Pretransplant sera without blood transfusion did not show any cytoxicity against Daudi cells. Although 42% of post-transplant sera showed cytotoxicity against Daudi cells, cytotoxic activity of the sera showed no relation with the occurrence of rejection episodes. Three serum samples of 5 patients whose grafts were removed due to rejection showed strong cytoxicity against Daudi cells and the sera of 2 other patients did not. There was no relation between the cytotoxicity against Daudi cells and antibodies against Epstein-Barr virus capsid antigen in the post-transplant sera.